澳洲墨尔本代写作业:光学成像和医学

2019-03-22 05:55

光学成像由于其高灵敏度、低成本、便携性和低辐射等优点，在生物医学研究中得到了广泛的应用。由于发光半导体纳米晶在有机染料上具有独特的光学和化学性质，量子点(QDs)已成为纳米技术中最令人兴奋的界面之一。量子点的独特性能使有力地研究分子、细胞和体内成像的优势包括窄,对称和可调发射光谱根据它们的大小和材料组成,广泛吸收光谱、量子产率高,极其photostable,更多的亮度和抗化学降解在这项研究中,我们制定一个theranostic nanocarrier在治疗癌症的靶向药物输送和成像。在此，通过将pluronic F-127、vitamin-E TPGS、TPGS-HER 2作为靶向部分，制备并表征了基于转基因的HER 2结合雷帕霉素和qd605负载CPNPs。通过不同的体外实验，取HER 2阳性和阴性细胞株，对我们制备的CPNPs进行治疗评价。结果证实，她的2个偶联雷帕霉素通过受体介导的内吞作用摄取的CPNPs比未偶联雷帕霉素和天然药物治疗的CPNPs更有效。此外，通过共聚焦显微镜对制备的多功能CPNPs的体外细胞成像研究进行了验证，结果表明，该多功能颗粒系统可以作为一颗子弹，为更好地治疗和诊断癌症提供一个新的概念。
澳洲墨尔本代写作业:光学成像和医学
Nowadays optical imaging has been explored in biomedical research due to its high sensitivity, cost effectiveness, portability and lack of radiation and it shows marvellous implication by providing information for both in vitro and in vivo studies. The development of light emitting semiconductor nanocrystals known as quantum dots (QDs) have emerged as one of the most exciting interface of nanotechnology due to its unique optical and chemical properties over organic dyes. The unique properties of these QDs lead powerfully to study in molecular, cellular and in vivo imaging with advantages including narrow, symmetrical and tunable emission spectra according to their size and material composition, broad absorption spectra, high quantum yield, extremely photostable, more brightness and resistance to chemical degradation .In this study, we have formulated a theranostic nanocarrier for targeted drug delivery and imaging in treatment of cancer. Here, preparation and characterisation of HER 2 conjugated rapamycin and QD 605 loaded CPNPs based on GMO was done by blending pluronic F-127, vitamin-E TPGS, TPGS-HER 2 as a targeting moiety. Therapeutic evaluation of our formulated CPNPs was performed by taking both HER 2 positive and negative cell line through different in vitro experiments. Results confirmed that HER 2 conjugated rapamycin loaded CPNPs due to their higher uptake through receptor mediated endocytosis were more effective than that of unconjugated CPNPs and native drug treated case. Furthermore, in vitro cellular imaging study of formulated multifunctional CPNPs was validated by confocal microscopy and the results suggested that this multifunctional particulate system can act as a single bullet capable of obtaining a new concept for better therapeutics and diagnostics in cancer.

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